Description
| Product Name: | Recombinant Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Protein |
| SKU: | RPCB0101 |
| Size: | 100µg |
| Tag: | C-His |
| Reactivity: | Influenza A |
| Expression Host: | Baculovirus-Insect Cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Protein , tested reactivity in Baculovirus-Insect Cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1.0 EU/μg of the protein by LAL method. |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0. Contact us for customized product form or formulation. |
| Gene ID: | 956529 |
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P03452 |
 | Recombinant Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin/HA Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60-65 kDa. |
