Description
| Product Name: | Recombinant Human Vimentin/VIM Protein |
| SKU: | RPCB1921 |
| Size: | 100µg |
| Tag: | C-His |
| Reactivity: | Human |
| Expression Host: | Baculovirus-Insect Cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Vimentin/VIM Protein , tested reactivity in Baculovirus-Insect Cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 40% acetonitrile. |
| Gene ID: | 7431 |
Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P08670 |
 | Recombinant Human Vimentin Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 55-70 kDa. |
