Description
| Product Name: | Recombinant Human TNF-α Protein (Gst Tag) |
| SKU: | RPES8100 |
| Size: | 20μg |
| Tag: | N-Gst |
| Reactivity: | Human |
| Expression Host: | E.coli |
| Accession: | P01375 |
| Sequence: | Val77-Leu233 |
| Calculated Molecular Weight: | 43 kDa |
| Observed Molecular Weight: | 45 kDa |
| Synonyms: | Tumor Necrosis Factor Ligand Superfamily Member, TNFSF, TNF, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, TNF alpha, Cachectin, Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, APC1, APC1 protein, DIF、TNF-alpha、TNFA、TNFSF2, Differentiation inducing factor, Macrophage cytotoxic factor, macrophage derived, member 2, Membrane Form, monocyte derived, soluble form, sTNFA, TNF superfamily, Tumor necrosis factor alpha |
| Endotoxin: | < 10 EU/mg of the protein as determined by the LAL method |
| Purity: | > 90% as determined by reducing SDS-PAGE. |
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1, CD120a, p55/60) and TNF-R2 (TNF receptor type 2, CD120b, p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
| Bio-activity: | Not validated for activity |
| Formulation: | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
