Description
| Product Name: | Recombinant Human IFN-alpha 10 Protein |
| SKU: | RPCB1414 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human IFN-alpha 10 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <1EU/μg of the protein by LAL method. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 3446 |
Interferons (IFN) are a family of cytokines with potent anti_x001E_viral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors . Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology. Structurally, type I IFNs belong to the class of five helical_x001E_bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds. Mature human IFNA10 shares 61% aa sequence identity with mouse IFNA7. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA10 has been shown to be a strong inducer of IFN-stimulated genes and anti_x001E_viral protection. Additionally, IFNA10 exhibits weak anti_x001E_viral effects against SARS-CoV-2 .
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P01566 |
 | Recombinant Human IFN-alpha 10/IFNA10 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-25kDa. |
 | Recombinant Human IFN-alpha 10/IFNA10 was measured in a cell cytotoxicity assay using TF-1 cells. The ED₅₀ for this effect is 0.26_x0000_1.04 ng/mL, corresponding to a specific activity of 9.62×105~3.85×106 units/mg. |
