Description
| Product Name: | Recombinant Human Complement C5A Protein |
| SKU: | RPCB1494 |
| Size: | 10µg |
| Tag: | NO-Tag |
| Reactivity: | Human |
| Expression Host: | E. coli |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Complement C5A Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.1EU/ug of the protein by LAL method. |
| Purity: | > 94 % by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 727 |
Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure . In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule . Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 . Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation . It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P01031 |
 | Recombinant Human Complement C5A Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 10-15kDa. |
