Description
system_update_altDatasheet
| Product Name: | CDK7 & CCNH & MNAT1 Recombinant Protein (RPES2393) |
| Product Code: | RPES2393 |
| Size: | 50 µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | CAK1,CDKN7,HCAK,MO15,p39MO15,STK1 |
| Accession: | P50613&P51946&P51948 |
| Sequence: | Ala 2-Phe 346&Tyr 2-Leu 323&Asp 2-Ser 309 |
| Fusion tag: | N-His |
| Activity: | The specific activity was determined to be 18 nmol/min/mg using MBP as substrate. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
| Purity: | > 85 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 118.8 (41.2 + 39.7 + 37.9) kDa |
| AP Mol Mass: | 25,38 & 44 kDa |
| Formulation: | Supplied as sterile PBS, pH 7.4 |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
