Description
| Product Name: | Recombinant Human CD3E&CD3G Protein |
| SKU: | RPCB0659 |
| Size: | 100µg |
| Tag: | C-hFc |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human CD3E&CD3G Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method |
| Purity: | > 95% by SDS-PAGE；> 95% by HPLC |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 916&917 |
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P07766(CD3E)&P09693(CD3G) |
 | Human CD3E&CD3G/CD3 epsilon&CD3 gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
 | The purity of Human CD3E&CD3G/CD3 Epsilon&CD3 Gamma was greater than 95% as determined by SEC-HPLC. |
 | Immobilized Tebentafusp, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Human CD3E&CD3G, hFc Tag with the EC50 of 0.22 μg/mL determined by ELISA. |
