Description
system_update_altDatasheet
| Product Name: | CD3E & CD3G Heterodimer Recombinant Protein (RPES2797) |
| Product Code: | RPES2797 |
| Size: | 20µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | CD3E, CD3G Heterodimer,IMD18,T3E,TCRE |
| Accession: | NP_000724.1&NP_000064.1 |
| Sequence: | Met 1-Asp126&Met1-Ser116 |
| Fusion tag: | |
| Activity: | Measured by its ability to bind OKT3-mIgG2a in a functional ELISA. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 77.2 (40.1+37.1) kDa |
| AP Mol Mass: | 42-47 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
