Description
| Product Name: | Recombinant Human CCL2/MCP-1 Protein |
| SKU: | RPCB1717 |
| Size: | 10µg |
| Tag: | NO-tag |
| Reactivity: | Human |
| Expression Host: | Pichia |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human CCL2/MCP-1 Protein , tested reactivity inPichiaand has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.1EU/μg |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 6347 |
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P13500 |
 | Recombinant Human CCL2/MCP-1 Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 10-15 kDa. Recombinant Human CCL2/MCP-1 chemoattract THP-1 cells. The ED₅₀ for this effect is typically 13.56-54.24 ng/mL. |
 | Recombinant Human CCL2/MCP-1 chemoattract THP-1 cells. The ED₅₀ for this effect is typically 13.56-54.24 ng/mL. |
