Description
| Product Name: | Rat NRP1 Recombinant Protein |
| Product Code: | RPPB0798 |
| Size: | 5µg |
| Species: | Rat |
| Target: | NRP1 |
| Synonyms: | Neuropilin-1, Vascular endothelial cell growth factor 165 receptor, CD304, Nrp1. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | NRP1 protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.� For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).� Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | FRSDKCGGTI KIENPGYLTS PGYPHSYHPS EKCEWLIQAP EPYQRIMINF NPHFDLEDRD CKYDYVEVID GENEGGRLWG KFCGKIAPSP VVSSGPFLFI KFVSDYETHG AGFSIRYEIF KRGPECSQNY TAPTGVIKSP GFPEKYPNSL ECTYIIFAPK MSEIILEFES FDLEQDSNPP GGVFCRYDRL EIWDGFPEVG PHIGRYCGQK TPGRIRSSSG ILSMVFYTDS AIAKEGFSAN YSVLQSSISE DFKCMEALGM ESGEIHSDQI TASSQYGTNW SVERSRLNYP ENGWTPGEDS YREWIQVDLG LLRFVTAVGT QGAISKETKK KYYVKTYRVD ISSNGEDWIT LKEGNKAIIF QGNTNPTDVV FGVFPKPLIT RFVRIKPASW ETGISMRFEV YGCKITDYPC SGMLGMVSGL ISDSQITASN QGDRNWMPEN IRLVTSRTGW ALPPSPHPYI NEWLQVDLGD EKIVRGVIIQ GGKHRENKVF MRKFKIAYSN NGSDWKMIMD DSKRKAKSFE GNNNYDTPEL RAFTPLSTRF IRIYPERATH SGLGLRMELL GCEVEVPTAG PTTPNGNPVD ECDDDQANCH SGTGDDFQLT GGTTVLATEK PTIIDSTIQS EFPTYGFNCE FGWGSHKTFC HWEHDSHAQL RWRVLTSKTG PIQDHTGDGN FIYSQADENQ KGKVARLVSP VVYSQSSAHC MTFWYHMSGS HVGTLRVKLH YQKPEEYDQL VWMVVGHQGD HWKEGRVLLH KSLKLYQVIF EGEIGKGNLG GIAVDDISIN NHIPQEDCAK PTDLDKKNTE IKIDETGSTP GYEEGKGDKN ISRKPGNVLK TLDPLEHHHH HH |
Neuropilin 1 (Nrp1) is a transmembrane glycoprotein which functions as a co-receptor for several extracellular ligands including class III/IV semaphorins, some isoforms of vascular endothelial growth factor and transforming growth factor beta. Nrp1 binds vascular endothelial growth factor (VEGF)-A and is believed to serve as a coreceptor for kinase insert domain-containing receptor (KDR) by connecting with KDR and enhancing VEGF signaling. Nrp1 is a marker of regulatory T cells.
NRP1 Rat Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 842 amino acids (22-855 a.a.) and having a molecular mass of 94.8kDa (Migrates at 100-150kDa on SDS-PAGE under reducing conditions).NRP1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Receptor, misc.Cellular Component: extracellular space; growth cone; cell surface; focal adhesion; cell soma; axon; early endosome; integral to membrane; plasma membrane; neurofilament; cytosolMolecular Function: heparin binding; vascular endothelial growth factor receptor activity; protein binding; semaphorin receptor activity; growth factor binding; metal ion bindingBiological Process: axon guidance; facial nerve structural organization; peptidyl-tyrosine phosphorylation; heart development; neuron migration; vestibulocochlear nerve structural organization; platelet-derived growth factor receptor signaling pathway; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; trigeminal nerve morphogenesis; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; positive chemotaxis; branchiomotor neuron axon guidance; response to wounding; dendrite development; angiogenesis; negative regulation of neuron apoptosis; trigeminal nerve structural organization; nervous system development; cell migration; axonal fasciculation; hepatocyte growth factor receptor signaling pathway; axon extension involved in axon guidance; patterning of blood vessels; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; otic placode formation; regulation of axon extension involved in axon guidance; positive regulation of axon extension involved in axon guidance; neuron development; artery morphogenesis; nerve development; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; retinal ganglion cell axon guidance |
| UniProt Protein Details: | |
| NCBI Summary: | transmembrane protein that acts as a receptor for semaphorin III; may play a role in neuronal development [RGD, Feb 2006] |
| UniProt Code: | Q9QWJ9 |
| NCBI GenInfo Identifier: | 9297002 |
| NCBI Gene ID: | 246331 |
| NCBI Accession: | Q9QWJ9.1 |
| UniProt Secondary Accession: | Q9QWJ9 |
| UniProt Related Accession: | Q9QWJ9 |
| Molecular Weight: | 103,082 Da |
| NCBI Full Name: | Neuropilin-1 |
| NCBI Synonym Full Names: | neuropilin 1 |
| NCBI Official Symbol: | Nrp1�� |
| NCBI Official Synonym Symbols: | Nrp�� |
| NCBI Protein Information: | neuropilin-1; vascular endothelial cell growth factor 165 receptor |
| UniProt Protein Name: | Neuropilin-1 |
| UniProt Synonym Protein Names: | Vascular endothelial cell growth factor 165 receptor; CD_antigen: CD304 |
| Protein Family: | Neuropilin |
| UniProt Gene Name: | Nrp1�� |
| UniProt Entry Name: | NRP1_RAT |
