Description
| Product Name: | Rat MIP 1a Recombinant Protein |
| Product Code: | RPPB1216 |
| Size: | 20µg |
| Species: | Rat |
| Target: | MIP 1a |
| Synonyms: | Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from 1 mg/ml solution containing no additives. |
| Solubility: | It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Tyr-Gly-Ala |
| Biological Activity: | The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml corresponding to a Specific Activity of 16,667IU/mg. |
Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6 and TNF-a from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.
Macrophage Inflammatory Protein-1 alpha Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. The MIP-1a is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin. |
| NCBI Summary: | mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006] |
| UniProt Code: | P50229 |
| NCBI GenInfo Identifier: | 1709024 |
| NCBI Gene ID: | 25542 |
| NCBI Accession: | P50229.1 |
| UniProt Secondary Accession: | P50229,A1EC87, |
| UniProt Related Accession: | P50229 |
| Molecular Weight: | 10,335 Da |
| NCBI Full Name: | C-C motif chemokine 3 |
| NCBI Synonym Full Names: | chemokine (C-C motif) ligand 3 |
| NCBI Official Symbol: | Ccl3�� |
| NCBI Official Synonym Symbols: | Scya3; MIP-1a�� |
| NCBI Protein Information: | C-C motif chemokine 3; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; macrophage inflammatory protein 1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3) |
| UniProt Protein Name: | C-C motif chemokine 3 |
| UniProt Synonym Protein Names: | Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Small-inducible cytokine A3 |
| Protein Family: | C-C motif chemokine |
| UniProt Gene Name: | Ccl3�� |
| UniProt Entry Name: | CCL3_RAT |
