Description
| Product Name: | Rat Midkine Recombinant Protein |
| Product Code: | RPPB0774 |
| Size: | 20µg |
| Species: | Rat |
| Target: | Midkine |
| Synonyms: | Midkine, MK, Mdk. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in 1xPBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Midkine Rat in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Midkine Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Midkine Rat should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD |
| Biological Activity: | The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml. |
Midkine (MK) is the product of a retinoic acid responsive gene, MK, and is a member of a family of heparin binding factors. It contains 121 amino acid residues including 10 conserved cysteine residues, all of which appear to be disulphide linked.Midkine is expressed during embryogenesis, showing an expression pattern that suggests functions in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm-epithelial interaction.The widespread downregulation of MK in the adult human is reverted in a number of cancers, in which polypeptides are able to act as both transforming growth factors and promoters of angiogenesis.Midkine (MK), induces chemotaxis of human neutrophils and was found to trigger mobilization of intracellular calcium of these cells.Midkine induces histamine release from rat peritoneal mast cells with a rapid response in a dose dependent manner.Midkine is also a potent stimulator of collagen and glycosaminoglycan synthesis.
Midkine Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.2kDa.The Midkine Rat is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (). |
| UniProt Protein Details: | |
| NCBI Summary: | heparin-binding growth factor; involved in early fetal development of the rat adrenal [RGD, Feb 2006] |
| UniProt Code: | Q9R1S9 |
| NCBI GenInfo Identifier: | 13540681 |
| NCBI Gene ID: | 81517 |
| NCBI Accession: | NP_110486.1 |
| UniProt Secondary Accession: | Q9R1S9 |
| UniProt Related Accession: | Q9R1S9 |
| Molecular Weight: | 15,406 Da |
| NCBI Full Name: | midkine |
| NCBI Synonym Full Names: | midkine |
| NCBI Official Symbol: | Mdk�� |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | midkine |
| UniProt Protein Name: | Midkine |
| UniProt Synonym Protein Names: | |
| Protein Family: | Midkine |
| UniProt Gene Name: | Mdk�� |
| UniProt Entry Name: |
