Description
| Product Name: | Rat IL6 Recombinant Protein |
| Product Code: | RPPB0531 |
| Size: | 5µg |
| Species: | Rat |
| Target: | IL6 |
| Synonyms: | IL6, Ifnb2, ILg6. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | IL6 protein solution (0.5mg/ml) contains 20% glycerol & Phosphate Buffered Saline (pH 7.4). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPFPTSQVR RGDFTEDTTH NRPVYTTSQV GGLITYVLRE ILEMRKELCN GNSDCMNSDDALSENNLKLP EIQRNDGCFQ TGYNQEICLL KICSGLLEFR FYLEFVKNNL QDNKKDKARVIQSNTETLVH IFKQEIKDSY KIVLPTPTSN ALLMEKLESQ KEWLRTKTIQ LILKALEEFL KVTMRSTRQT HHHHHH |
| Biological Activity: | Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED50 for this effects is less or equal to 0.1 ng/ml. |
Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
IL6 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 196 amino acids (25-211 a.a.) and having a molecular mass of 22.8kDa (Molecular size on SDS-PAGE will appear at approximately 18-28 kDa).IL6 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. |
| NCBI Summary: | a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006] |
| UniProt Code: | P20607 |
| NCBI GenInfo Identifier: | 124350 |
| NCBI Gene ID: | 24498 |
| NCBI Accession: | P20607.1 |
| UniProt Related Accession: | P20607 |
| Molecular Weight: | 24,357 Da |
| NCBI Full Name: | Interleukin-6 |
| NCBI Synonym Full Names: | interleukin 6 |
| NCBI Official Symbol: | Il6�� |
| NCBI Official Synonym Symbols: | ILg6; Ifnb2�� |
| NCBI Protein Information: | interleukin-6; IL-6; Interleukin 6 (interferon, beta 2) |
| UniProt Protein Name: | Interleukin-6 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il6�� |
| UniProt Entry Name: | IL6_RAT |
