Description
| Product Name: | Rat IL 6 Recombinant Protein |
| Product Code: | RPPB0530 |
| Size: | 10µg |
| Species: | Rat |
| Target: | IL 6 |
| Synonyms: | IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from 0.02% TFA. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin-6 with 10mM HCl at a concentration of 0.1 mg/ml which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser |
| Biological Activity: | The ED50 is less than 0.01 ng/ml as determined by dose-dependent stimulation of mouse 7TD1 cells. |
Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
Interleukin-6 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton. The IL6 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. |
| NCBI Summary: | a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006] |
| UniProt Code: | P20607 |
| NCBI GenInfo Identifier: | 124350 |
| NCBI Gene ID: | 24498 |
| NCBI Accession: | P20607.1 |
| UniProt Related Accession: | P20607 |
| Molecular Weight: | 24,357 Da |
| NCBI Full Name: | Interleukin-6 |
| NCBI Synonym Full Names: | interleukin 6 |
| NCBI Official Symbol: | Il6�� |
| NCBI Official Synonym Symbols: | ILg6; Ifnb2�� |
| NCBI Protein Information: | interleukin-6; IL-6; Interleukin 6 (interferon, beta 2) |
| UniProt Protein Name: | Interleukin-6 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il6�� |
| UniProt Entry Name: | IL6_RAT |
