Description
| Product Name: | Rat IL 33 Recombinant Protein |
| Product Code: | RPPB0668 |
| Size: | 20µg |
| Species: | Rat |
| Target: | IL 33 |
| Synonyms: | Interleukin-33, IL-33. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | IL 33 protein solution (0.5mg/ml) containing Phosphate BufferedSaline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrierprotein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHHSSGLVPRGSH MGSSIQGTSLLTESCALSTY NDQSVSFVLE NGCYVINVED CGKNQEKDKV LLRYYESSFP AQSGDGVDGK KLMVNMSPIKDTDIWLNAND KDYSVELQKG DVSPPDQAFF VLHKKSSDFV SFECKNLPGT YIGVKDNQLA LVEENDESCNNIMFKLSKM |
nterleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
IL 33 Rat Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 179 amino acids (109-264 a.a) andhaving a molecular mass of 19.8kDa.IL 33 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q66H70 |
| NCBI GenInfo Identifier: | 62079057 |
| NCBI Gene ID: | 361749 |
| NCBI Accession: | NP_001014188.1 |
| UniProt Secondary Accession: | Q66H70 |
| UniProt Related Accession: | Q66H70 |
| Molecular Weight: | 29,486 Da |
| NCBI Full Name: | interleukin-33 |
| NCBI Synonym Full Names: | interleukin 33 |
| NCBI Official Symbol: | Il33�� |
| NCBI Official Synonym Symbols: | RGD1311155�� |
| NCBI Protein Information: | interleukin-33; IL-33 |
| UniProt Protein Name: | Interleukin-33 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il33�� |
| UniProt Entry Name: | IL33_RAT |
