Description
| Product Name: | Rat GRO a Recombinant Protein |
| Product Code: | RPPB1145 |
| Size: | 25µg |
| Species: | Rat |
| Target: | GRO a |
| Synonyms: | Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha(1-73), chemokine (C-X-C motif) ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2?m filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, and 150mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized CXCL1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized CXCL1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK |
| Biological Activity: | Determined by its ability to chemoattract rat neutrophils using a concentration range of 10-100ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg. |
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-?). It is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines.
CXCL1 Rat Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7.8 kDa. The Rat CXCL1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis Cellular Component: extracellular space Molecular Function:growth factor activity; chemokine activity; CXCR chemokine receptor binding Biological Process: neutrophil chemotaxis; response to glucocorticoid stimulus; response to amphetamine; positive regulation of potassium ion transport; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; response to estradiol stimulus; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to heat; response to molecule of bacterial origin; positive regulation of superoxide release; response to gamma radiation; immune response; acute inflammatory response |
| NCBI Summary: | acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response [RGD, Feb 2006] |
| UniProt Code: | P14095 |
| NCBI GenInfo Identifier: | 1170021 |
| NCBI Gene ID: | 81503 |
| NCBI Accession: | P14095.2 |
| UniProt Related Accession: | P14095 |
| Molecular Weight: | 10,249 Da |
| NCBI Full Name: | Growth-regulated alpha protein |
| NCBI Synonym Full Names: | chemokine (C-X-C motif) ligand 1 |
| NCBI Official Symbol: | Cxcl1�� |
| NCBI Official Synonym Symbols: | Gro1; CINC-1�� |
| NCBI Protein Information: | growth-regulated alpha protein; C-X-C motif chemokine 1; cytokine-induced neutrophil chemoattractant 1; platelet-derived growth factor-inducible protein KC; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) |
| UniProt Protein Name: | Growth-regulated alpha protein |
| UniProt Synonym Protein Names: | C-X-C motif chemokine 1; Cytokine-induced neutrophil chemoattractant 1; CINC-1; Platelet-derived growth factor-inducible protein KC |
| Protein Family: | Growth-regulated alpha protein |
| UniProt Gene Name: | Cxcl1�� |
| UniProt Entry Name: | GROA_RAT |
