Description
| Product Name: | Rat Fractalkine Recombinant Protein |
| Product Code: | RPPB1138 |
| Size: | 20µg |
| Species: | Rat |
| Target: | Fractalkine |
| Synonyms: | Fractalkine, CX3CL1, Neurotactin, CX3C membrane-anchored chemokine, Small inducible cytokine D1, NTN, NTT, CXC3, CXC3C, SCYD1, ABCD-3, C3Xkine. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Fractalkine in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized FractalkineRat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fractalkine should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNG |
| Biological Activity: | Determined by its ability to chemoattract human monocytes using a concentration range of 5.0-10.0 ng/ml, corresponding to a specific activity of 100,000-200,000units/mg. |
Fractalkine soluble form is chemotactic for t-cells and monocytes, but not for neutrophils. Fractalkine membrane-bound form promotes adhesion of those leukocytes to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373-amino acid) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the cell surface. Fractalkine gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.
Fractalkine Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8.7kDa.The Fractalkine is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. |
| NCBI Summary: | chemokine that binds its receptor CX3CR1; induces chemotaxis and increased intracellular calcium levels in microglia [RGD, Feb 2006] |
| UniProt Code: | O55145 |
| NCBI GenInfo Identifier: | 18266784 |
| NCBI Gene ID: | 89808 |
| NCBI Accession: | O55145.3 |
| UniProt Related Accession: | O55145 |
| Molecular Weight: | 41,977 Da |
| NCBI Full Name: | Fractalkine |
| NCBI Synonym Full Names: | chemokine (C-X3-C motif) ligand 1 |
| NCBI Official Symbol: | Cx3cl1�� |
| NCBI Official Synonym Symbols: | Cx3c; Scyd1�� |
| NCBI Protein Information: | fractalkine; neurotactin; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D, 1 |
| UniProt Protein Name: | Fractalkine |
| UniProt Synonym Protein Names: | C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1Cleaved into the following chain:Processed fractalkine |
| Protein Family: | Fractalkine |
| UniProt Gene Name: | Cx3cl1�� |
| UniProt Entry Name: | X3CL1_RAT |
