Description
| Product Name: | Rat FGF 2 Recombinant Protein |
| Product Code: | RPPB0213 |
| Size: | 50µg |
| Species: | Rat |
| Target: | FGF 2 |
| Synonyms: | HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b, Fibroblast Growth Factor-basic, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | FGF-b was lyophilized from 1mg/ml solution after extensive dialysis against 10mM Na2PO4, pH 7.5 and 50mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Rat bFGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat FGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MPALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS |
| Biological Activity: | The ED50 range as determined by the dose-dependant proliferation of 3T3 cells was found to be less than 0.13-0.2ng/ml. |
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.
bFGF Rat Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.4 kDa.The bFGF2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Activator; Motility/polarity/chemotaxis Cellular Component: extracellular space; cytoplasm; cytosol; nucleus Molecular Function:heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; fibroblast growth factor receptor binding; chemoattractant activity Biological Process: activation of MAPKK activity; wound healing; regulation of cell cycle; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; adrenocorticotropin hormone secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; embryonic development ending in birth or egg hatching; negative regulation of cell proliferation; glial cell differentiation; substantia nigra development; positive chemotaxis; induction of an organ; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; response to axon injury; angiogenesis; aging; positive regulation of cardiac muscle cell proliferation; positive regulation of granule cell precursor proliferation; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; stem cell development; cell migration during sprouting angiogenesis; ureteric bud branching; positive regulation of cell division; release of sequestered calcium ion into cytosol; phosphatidylinositol biosynthetic process; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of cell differentiation; inositol phosphate biosynthetic process; positive regulation of epithelial cell proliferation; lung development |
| NCBI Summary: | activates the MAP kinase signaling pathway; plays a role in synaptic transmission; induces cell proliferation [RGD, Feb 2006] |
| UniProt Code: | P13109 |
| NCBI GenInfo Identifier: | 122745 |
| NCBI Gene ID: | 54250 |
| NCBI Accession: | P13109.1 |
| UniProt Related Accession: | P13109 |
| Molecular Weight: | 17,139 Da |
| NCBI Full Name: | Fibroblast growth factor 2 |
| NCBI Synonym Full Names: | fibroblast growth factor 2 |
| NCBI Official Symbol: | Fgf2�� |
| NCBI Official Synonym Symbols: | bFGF; Fgf-2�� |
| NCBI Protein Information: | fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2 |
| UniProt Protein Name: | Fibroblast growth factor 2 |
| UniProt Synonym Protein Names: | Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2 |
| Protein Family: | Fibroblast growth factor |
| UniProt Gene Name: | Fgf2�� |
| UniProt Entry Name: | FGF2_RAT |
