Description
system_update_altDatasheet
Rat FasL PharmaGenie ELISA Kit
| Product SKU: | SBRS1633 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha. |
| Uniprot: | P36940 |
| Gene ID: | 25385 |
| Gene Names: | CD95L / FASL / FASLG / TNFSF6 |
| Synonyms: | Tumor necrosis factor ligand superfamily member 6 (CD95 ligand) (CD95-L) (Fas antigen ligand) (Fas ligand) (FasL) (CD antigen CD178) [Cleaved into: Tumor necrosis factor ligand superfamily member 6, membrane form Tumor necrosis factor ligand superfamily member 6, soluble form (Receptor-binding FasL ectodomain) (Soluble Fas ligand) (sFasL) ADAM10-processed FasL form (APL) FasL intracellular domain (FasL ICD) (SPPL2A-processed FasL form) (SPA)] |
| Target Species: | Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 90 pg/ml - 60000 pg/ml |
| Sensitivity: | 90 pg/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Rat FasL PharmaGenie ELISA Kit (SBRS1633) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
