Description
| Product Name: | Rat BD 4 Recombinant Protein |
| Product Code: | RPPB0077 |
| Size: | 20µg |
| Species: | Rat |
| Target: | BD 4 |
| Synonyms: | Beta-defensin 4, BD-4, BD-2, Defensin, beta 4, RBD-2, RBD-4, Defb4, Defb2, Defb3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | BD-4 protein was lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized BD-4 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized BD-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K |
| Biological Activity: | Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 5-50�g/ml. |
Defensins are cationic peptides with a large spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. 4 human Beta-defensins have been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.
BD-4 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.4kDa.The BD-4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells. |
| NCBI Summary: | cationic peptides with broad-spectrum antimicrobial activity; may mediate mucosal defenses of several organs [RGD, Feb 2006] |
| UniProt Code: | O88514 |
| NCBI GenInfo Identifier: | 25742583 |
| NCBI Gene ID: | 64389 |
| NCBI Accession: | NP_071989.1 |
| UniProt Secondary Accession: | O88514,Q32ZE9, |
| UniProt Related Accession: | O88514 |
| Molecular Weight: | |
| NCBI Full Name: | beta-defensin 4 |
| NCBI Synonym Full Names: | defensin beta 4 |
| NCBI Official Symbol: | Defb4�� |
| NCBI Official Synonym Symbols: | Defb2; Defb3�� |
| NCBI Protein Information: | beta-defensin 4 |
| UniProt Protein Name: | Beta-defensin 4 |
| UniProt Synonym Protein Names: | BD-2; Defensin, beta 4; RBD-2; RBD-4 |
| UniProt Gene Name: | Defb4�� |
