Description
system_update_altDatasheet
Rat 4-1BB/TNFRSF9/CD137 PharmaGenie ELISA Kit
| Product SKU: | SBRS1603 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with the following cytokines tested: Rat Activin A, Adiponectin (ACRP30), CD80 (B7-1), CD48 (SLAMF2), CTACK (CCL27), Decorin, Eotaxin-1 (CCL11), EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1 (GDNF R), gp130, HGF, IL-1 R6 (IL-1 Rrp2), IL-1 ra (IL-1 F3), IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A (CD321/F11R), MIP-1 alpha (CCL3), Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES (CCL5), SCF, TIM1 (KIM-1), TIMP-2, TREM-1, TWEAK R (TNFRSF12). |
| Uniprot: | Q4W8J3 |
| Synonyms: | 4-1BB homolog |
| Target Species: | Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 1.5 ng/ml - 300 ng/ml |
| Sensitivity: | 1.5 ng/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Rat 4-1BB/TNFRSF9/CD137 PharmaGenie ELISA Kit (SBRS1603) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
