Description
| Product Name: | Rabbit IGF1 Recombinant Protein |
| Product Code: | RPPB0427 |
| Size: | 50µg |
| Species: | Rabbit |
| Target: | IGF1 |
| Synonyms: | Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (0.5mg/ml) solution with 0.02% NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized rbIGF-I in sterile 0.4% NaHCO3 adjusted to ph 8-9, not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% �as determined by:(a) Gel-filtration chromatography under non denaturing conditions.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MTAGPETLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKP |
| Biological Activity: | IGF-I is biologically active when compared to human IGF-1. The ED50, calculated by the dose -dependent proliferation of human MCF/7 cells is 5 to 25ng/ml in the cell culture mixture dependent on culture conditions. Its activity consists of 30-40 % compared to human IGF-1. |
The IGFs comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone. Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin'. Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A, and somatomedin B. IGF-1 is a small protein secreted mostly but not exclusively by the liver and circulating in blood mostly as a complex with several IGF binding proteins. It has growth-regulating, insulin-like, and mitogenic functions and it is secreted in response to growth hormone stimulation.
Insulin-Like Growth Factor-I Rabbit Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7639 Dalton.�
| UniProt Protein Function: | Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation |
| UniProt Code: | Q95222 |
| NCBI GenInfo Identifier: | 126722801 |
| NCBI Gene ID: | 100008668 |
| NCBI Accession: | NP_001075495.1 |
| UniProt Secondary Accession: | Q95222,O18846, |
| UniProt Related Accession: | Q95222 |
| Molecular Weight: | 16,092 Da |
| NCBI Full Name: | insulin-like growth factor I |
| NCBI Official Symbol: | IGF1�� |
| NCBI Official Synonym Symbols: | IGF-IB�� |
| NCBI Protein Information: | insulin-like growth factor I; IGF-I; somatomedin; insulin-like growth factor IB |
| UniProt Protein Name: | Insulin-like growth factor I |
| UniProt Synonym Protein Names: | Somatomedin |
| Protein Family: | Insulin-like growth factor |
| UniProt Gene Name: | IGF1�� |
| UniProt Entry Name: | IGF1_RABIT |
