Description
| Product Name: | PRG4 Rabbit Polyclonal Antibody |
| SKU: | CAB15379 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1264-1363 of human PRG4 (NP_001121180.2). |
| Sequence: | ERAI GPSQ THTI RIQY SPAR LAYQ DKGV LHNE VKVS ILWR GLPN VVTS AISL PNIR KPDG YDYY AFSK DQYY NIDV PSRT ARAI TTRS GQTL SKVW YNCP |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:200 - 1:2000 |
| Synonyms: | MSF; SZP; CACP; HAPO; JCAP; PRG4 |
| Positive Sample: | LO2 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Secreted. |
| Calculated MW: | 151kDa |
| Observed MW: | 150kDa |
The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants.
| Purification Method: | Affinity purification |
| Gene ID: | 10216 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from LO2 cells, using PRG4 Rabbit pAb (CAB15379) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
