Description
| 抗体名: | PPP2R2A Antibody (PACO51846) |
| 抗体コード: | PACO51846 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| 反応性: | Human, Mouse, Rat |
| 免疫原: | Recombinant Human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B α isoform protein (2-149AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat brain tissue, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue. All lanes: PPP2R2A antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 53 kDa. Observed band size: 52 kDa.. |
 | Immunofluorescent analysis of Hela cells using PACO51846 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human melanoma using PACO51846 at dilution of 1:100. |
| バックグラウンド: | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. |
| シノニム: | Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B α isoform (PP2A subunit B isoform B55-alpha) (PP2A subunit B isoform PR55-alpha) (PP2A subunit B isoform R2-alpha) (PP2A subunit B isoform alpha), PPP2R2A |
| UniProt Protein Function: | PPP2R2A: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with TP53. Expressed in all tissues examined. Belongs to the phosphatase 2A regulatory subunit B family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 8p21.2 Cellular Component: cytosol; nucleoplasm; protein phosphatase type 2A complex Molecular Function:protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity Biological Process: G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation |
| NCBI Summary: | The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
| UniProt Code: | P63151 |
| NCBI GenInfo Identifier: | 52783535 |
| NCBI Gene ID: | 5520 |
| NCBI Accession: | P63151.1 |
| UniProt Secondary Accession: | P63151,P50409, Q00007, B2RBU8, B4E1T7, |
| UniProt Related Accession: | P63151 |
| Molecular Weight: | 53,000 Da |
| NCBI Full Name: | Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform |
| NCBI Synonym Full Names: | protein phosphatase 2 regulatory subunit Balpha |
| NCBI Official Symbol: | PPP2R2A |
| NCBI Official Synonym Symbols: | B55A; PR52A; PR55A; B55ALPHA |
| NCBI Protein Information: | serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform |
| UniProt Protein Name: | Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform |
| UniProt Synonym Protein Names: | PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B isoform alpha |
| Protein Family: | Serine/threonine-protein phosphatase |
| UniProt Gene Name: | PPP2R2A |
| UniProt Entry Name: | 2ABA_HUMAN |
