Description
| 抗体名: | POU5F1B Antibody (PACO59029) |
| 抗体コード: | PACO59029 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Putative POU domain, class 5, transcription factor 1B protein (10-138AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate. All lanes: POU5F1B antibody at 6.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa. |
 | IHC image of PACO59029 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO59029 diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Shows weak transcriptional activator activity. |
| シノニム: | Putative POU domain, class 5, transcription factor 1B (Oct4-pg1) (Octamer-binding protein 3-like) (Octamer-binding transcription factor 3-like), POU5F1B, OCT4PG1 OTF3C OTF3P1 POU5F1P1 POU5FLC20 POU5FLC8 |
| UniProt Protein Function: | POU5F1B: Shows weak transcriptional activator activity. Belongs to the POU transcription factor family. Class- 5 subfamily. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 8q24.21 Molecular Function:transcription factor activity |
| NCBI Summary: | This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development. [provided by RefSeq, Apr 2009] |
| UniProt Code: | Q06416 |
| NCBI GenInfo Identifier: | 357528826 |
| NCBI Gene ID: | 5462 |
| NCBI Accession: | Q06416.2 |
| UniProt Secondary Accession: | Q06416,D5K9S4, D5K9S9, D5K9T0, D5K9T1, D5K9T3, D5K9U3 D5K9U6, D5K9V4, D5K9V6, D5K9W0, D5K9W2, |
| UniProt Related Accession: | Q06416 |
| Molecular Weight: | 38,588 Da |
| NCBI Full Name: | Putative POU domain, class 5, transcription factor 1B |
| NCBI Synonym Full Names: | POU class 5 homeobox 1B |
| NCBI Official Symbol: | POU5F1B |
| NCBI Official Synonym Symbols: | OTF3C; OTF3P1; OCT4PG1; OCT4-PG1; POU5F1P1; POU5F1P4; POU5FLC8; POU5FLC20 |
| NCBI Protein Information: | putative POU domain, class 5, transcription factor 1B |
| UniProt Protein Name: | Putative POU domain, class 5, transcription factor 1B |
| UniProt Synonym Protein Names: | Oct4-pg1; Octamer-binding protein 3-like; Octamer-binding transcription factor 3-like |
| Protein Family: | Putative POU domain, class 5, transcription factor |
| UniProt Gene Name: | POU5F1B |
| UniProt Entry Name: | P5F1B_HUMAN |
| Antibodies |
| Anti-POU5F1B Antibody (CAB15705) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
