Description
| Product Name: | Porcine Leptin Recombinant Protein |
| Product Code: | RPPB0713 |
| Size: | 100µg |
| Species: | Porcine |
| Target: | Leptin |
| Synonyms: | OB Protein, Obesity Protein, OBS, Obesity factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized recombinant porcine leptin in sterile 0.4% NaHCO3 adjusted to ph 8-9, not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Gel filtration analysis.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Trp |
| Biological Activity: | Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
Leptin Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and additional Ala at N-terminus, having a molecular mass of 16kDa. The Leptin is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. |
| UniProt Code: | Q29406 |
| NCBI GenInfo Identifier: | 55741433 |
| NCBI Gene ID: | 396832 |
| NCBI Accession: | NP_999005.1 |
| UniProt Secondary Accession: | Q29406,O19095, Q95251, |
| UniProt Related Accession: | Q29406 |
| Molecular Weight: | 18,661 Da |
| NCBI Full Name: | leptin |
| NCBI Official Symbol: | LEP�� |
| NCBI Official Synonym Symbols: | OB; OBS�� |
| NCBI Protein Information: | leptin; obese; obesity factor |
| UniProt Protein Name: | Leptin |
| UniProt Synonym Protein Names: | Obesity factor |
| Protein Family: | Leptin |
| UniProt Gene Name: | LEP�� |
| UniProt Entry Name: | LEP_PIG |
