Description
| Product Name: | Porcine IL 1 beta Recombinant Protein |
| Product Code: | RPPB0463 |
| Size: | 10µg |
| Species: | Porcine |
| Target: | IL 1 beta |
| Synonyms: | Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The IL 1 beta was lyophilized from a 0.2�m filtered concentrated solution in PBS pH 7.4, containing 3 % trehalose. |
| Solubility: | It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL 1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL 1 beta should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP |
| Biological Activity: | The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 � 105 IU/mg. |
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Recombinant IL 1 beta Porcine produced in E.coli cells is a non-glycosylated, homodimeric protein containing 153 amino acid chain and having a molecular mass of 17.6kDa. The IL 1 beta is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
| UniProt Code: | P26889 |
| NCBI GenInfo Identifier: | 47522926 |
| NCBI Gene ID: | 397122 |
| NCBI Accession: | NP_999220.1 |
| UniProt Related Accession: | P26889 |
| Molecular Weight: | 30,404 Da |
| NCBI Full Name: | interleukin-1 beta |
| NCBI Official Symbol: | IL1B�� |
| NCBI Protein Information: | interleukin-1 beta; IL-1 beta; prointerleukin-1 beta |
| UniProt Protein Name: | Interleukin-1 beta |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL1B�� |
| UniProt Entry Name: | IL1B_PIG |
