Description
| Product Name: | Porcine GMCSF Recombinant Protein |
| Product Code: | RPPB0361 |
| Size: | 100µg |
| Species: | Porcine |
| Target: | GMCSF |
| Synonyms: | CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a sterile (0.2�m) filtered solution containing 10mM sodium phosphate, pH 7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized GMCSF in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAPTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK |
| Biological Activity: | The ED50, as determined by TF-1 cell proliferation is 4.52ng/ml corresponding to a specific activity which is 2.2 x 10^5 units/mg. |
GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of GMCSF is found extracellularly as a homodimer. GMCSF has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Granulocyte Macrophage-Colony Stimulating Factor Porcine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 128 amino acids and having a� molecular mass of 14.5kDa.GMCSF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family. |
| UniProt Protein Details: | Protein type:Secreted; Cytokine; Secreted, signal peptide Cellular Component: extracellular space Molecular Function:growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; positive regulation of interleukin-23 production; myeloid dendritic cell differentiation; regulation of cell proliferation; epithelial cell proliferation; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of gene expression; positive regulation of cell proliferation; immune response; positive regulation of macrophage differentiation; positive regulation of DNA replication |
| NCBI Summary: | plays a role in alveolar epithelial fluid transport [RGD, Feb 2006] |
| UniProt Code: | P48750 |
| NCBI GenInfo Identifier: | 215273996 |
| NCBI Gene ID: | 116630 |
| NCBI Accession: | P48750.2 |
| UniProt Related Accession: | P48750 |
| Molecular Weight: | 16,562 Da |
| NCBI Full Name: | Granulocyte-macrophage colony-stimulating factor |
| NCBI Synonym Full Names: | colony stimulating factor 2 (granulocyte-macrophage) |
| NCBI Official Symbol: | Csf2�� |
| NCBI Official Synonym Symbols: | Gmcsf; Gm-csf�� |
| NCBI Protein Information: | granulocyte-macrophage colony-stimulating factor; CSF |
| UniProt Protein Name: | Granulocyte-macrophage colony-stimulating factor |
| UniProt Synonym Protein Names: | Colony-stimulating factor; CSF |
| Protein Family: | Granulocyte-macrophage colony-stimulating factor |
| UniProt Gene Name: | Csf2�� |
| UniProt Entry Name: | CSF2_RAT |
