Description
| 抗体名: | PITPNA Antibody (PACO63691) |
| 抗体コード: | PACO63691 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Phosphatidylinositol transfer protein α isoform protein (1-270AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | Antigen Affinity Purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63691 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of U251 cells with PACO63691 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| バックグラウンド: | Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes. |
| シノニム: | Phosphatidylinositol transfer protein α isoform (PI-TP-alpha) (PtdIns transfer protein alpha) (PtdInsTP alpha), PITPNA, PITPN |
| UniProt Protein Function: | PITPNA: Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes. Belongs to the PtdIns transfer protein family. PI transfer class I subfamily. |
| UniProt Protein Details: | Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 17p13.3 Molecular Function:phosphatidylcholine transmembrane transporter activity; phosphatidylinositol transporter activity Biological Process: visual perception |
| NCBI Summary: | This gene encodes a member of a family of lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces. The protein is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) by phosphoinositide-3-kinase.[provided by RefSeq, Sep 2009] |
| UniProt Code: | Q00169 |
| NCBI GenInfo Identifier: | 130770 |
| NCBI Gene ID: | 5306 |
| NCBI Accession: | Q00169.2 |
| UniProt Secondary Accession: | Q00169,P16446, P48738, P53810, Q2HJ54, |
| UniProt Related Accession: | Q00169 |
| Molecular Weight: | 31,806 Da |
| NCBI Full Name: | Phosphatidylinositol transfer protein alpha isoform |
| NCBI Synonym Full Names: | phosphatidylinositol transfer protein alpha |
| NCBI Official Symbol: | PITPNA |
| NCBI Official Synonym Symbols: | PITPN; VIB1A; HEL-S-36; PI-TPalpha |
| NCBI Protein Information: | phosphatidylinositol transfer protein alpha isoform |
| UniProt Protein Name: | Phosphatidylinositol transfer protein alpha isoform |
| Protein Family: | Phosphatidylinositol transfer protein |
| UniProt Gene Name: | PITPNA |
| UniProt Entry Name: | PIPNA_HUMAN |
