Description
| 抗体名: | Phospho-RPS6KA4 (Thr568) Antibody (PACO24430) |
| 抗体コード: | PACO24430 |
| サイズ: | 100ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
| 反応性: | Human, Mouse |
| 免疫原: | Peptide sequence around phosphorylation site of threonine 568 (M-Q-T(p)-P-C) derived from Human MSK2. |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using MSK2 (Phospho-Thr568) antibody. The lane on the right is treated with the synthesized peptide. |
 | Immunohistochemistry analysis of paraffin-embedded human brain tissue using MSK2 (Phospho-Thr568) antibody. The picture on the right is treated with the synthesized peptide. |
| バックグラウンド: | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. |
| シノニム: | EC 2.7.11.1; kinase MSK2; KS6A4; Ribosomal protein kinase B; RPS6KA4; RSK-B; Similar to ribosomal protein S6 kinase; 90kD; polypeptide 4 |
| UniProt Protein Function: | MSK2: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Phosphorylates H3 and HMG-14 in response to growth factors and cellular stress. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; AGC group; RSK family; MSK subfamily Chromosomal Location of Human Ortholog: 11q11-q13 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; histone serine kinase activity (H3-S10 specific); mitogen-activated protein kinase p38 binding; protein binding; protein serine/threonine kinase activity Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; histone phosphorylation; negative regulation of cytokine production; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| UniProt Code: | O75676 |
| NCBI GenInfo Identifier: | 37999483 |
| NCBI Gene ID: | 8986 |
| NCBI Accession: | O75676.1 |
| UniProt Secondary Accession: | O75676,O75585, Q53ES8, A8K7Z8, |
| UniProt Related Accession: | O75676 |
| Molecular Weight: | 84,884 Da |
| NCBI Full Name: | Ribosomal protein S6 kinase alpha-4 |
| NCBI Synonym Full Names: | ribosomal protein S6 kinase A4 |
| NCBI Official Symbol: | RPS6KA4 |
| NCBI Official Synonym Symbols: | MSK2; RSK-B; S6K-alpha-4 |
| NCBI Protein Information: | ribosomal protein S6 kinase alpha-4 |
| UniProt Protein Name: | Ribosomal protein S6 kinase alpha-4 |
| UniProt Synonym Protein Names: | 90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; RSKB |
| Protein Family: | Ribosomal protein S6 kinase |
| UniProt Gene Name: | RPS6KA4 |
| UniProt Entry Name: | KS6A4_HUMAN |
| Antibodies | ELISA Kits |
| Anti-RPS6KA4 Antibody (CAB10130) | MSK2 Colorimetric Cell-Based ELISA Kit |
| Phospho-RPS6KA4 (T568) Antibody (PACO03171) | |
| RPS6KA4 Antibody (PACO05658) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
