Description
| 抗体名: | NOXO1 Antibody (PACO59816) |
| 抗体コード: | PACO59816 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | Recombinant Human NADPH oxidase organizer 1 protein (304-376AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59816 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO59816 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59816 diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity. |
| シノニム: | NADPH oxidase organizer 1 (NADPH oxidase regulatory protein) (Nox organizer 1) (Nox-organizing protein 1) (SH3 and PX domain-containing protein 5), NOXO1, P41NOX SH3PXD5 |
| UniProt Protein Function: | NOXO1: Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: NADPH oxidase complex; plasma membrane Molecular Function:enzyme binding; identical protein binding; phospholipid binding; protein binding; superoxide-generating NADPH oxidase activator activity Biological Process: extracellular matrix disassembly; regulation of hydrogen peroxide metabolic process |
| NCBI Summary: | This gene encodes an NADPH oxidase (NOX) organizer, which positively regulates NOX1 and NOX3. The protein contains a PX domain and two SH3 domains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012] |
| UniProt Code: | Q8NFA2 |
| NCBI GenInfo Identifier: | 74751269 |
| NCBI Gene ID: | 124056 |
| NCBI Accession: | Q8NFA2.1 |
| UniProt Secondary Accession: | Q8NFA2,Q86YM1, Q8NFA3, Q96B73, |
| UniProt Related Accession: | Q8NFA2 |
| Molecular Weight: | 40,668 Da |
| NCBI Full Name: | NADPH oxidase organizer 1 |
| NCBI Synonym Full Names: | NADPH oxidase organizer 1 |
| NCBI Official Symbol: | NOXO1 |
| NCBI Official Synonym Symbols: | SNX28; P41NOX; P41NOXA; P41NOXB; P41NOXC; SH3PXD5 |
| NCBI Protein Information: | NADPH oxidase organizer 1 |
| UniProt Protein Name: | NADPH oxidase organizer 1 |
| UniProt Synonym Protein Names: | NADPH oxidase regulatory protein; Nox organizer 1; Nox-organizing protein 1; SH3 and PX domain-containing protein 5 |
| Protein Family: | NADPH oxidase organizer |
| UniProt Gene Name: | NOXO1 |
| UniProt Entry Name: | NOXO1_HUMAN |
| Antibodies |
| Anti-NOXO1 Antibody (CAB10477) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
