Description
| 抗体名: | NDUFB8 Antibody (PACO57888) |
| 抗体コード: | PACO57888 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
| 反応性: | Human, Mouse, Rat |
| 免疫原: | Recombinant Human NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 8, mitochondrial protein (29-132AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: NIH/3T3 whole cell lysate, Rat brain tissue, Mouse heart tissue. All lanes: NDUFB8 antibody at 2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22, 21, 19 kDa. Observed band size: 22 kDa. |
 | IHC image of PACO57888 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO57888 diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| バックグラウンド: | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| シノニム: | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 8, mitochondrial (Complex I-ASHI) (CI-ASHI) (NADH-ubiquinone oxidoreductase ASHI subunit), NDUFB8 |
| UniProt Protein Function: | NDUFB8: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB8 subunit family.Protein type: Endoplasmic reticulum; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.99.3; Membrane protein, integral; Mitochondrial; EC 1.6.5.3Chromosomal Location of Human Ortholog: 10q24.31Cellular Component: endoplasmic reticulum; mitochondrial inner membrane; integral to membrane; mitochondrial respiratory chain complex IMolecular Function: NADH dehydrogenase (ubiquinone) activityBiological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | O95169 |
| NCBI GenInfo Identifier: | 6093490 |
| NCBI Gene ID: | 4714 |
| NCBI Accession: | O95169.1 |
| UniProt Secondary Accession: | O95169 |
| UniProt Related Accession: | O95169 |
| Molecular Weight: | |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase subunit B8 |
| NCBI Official Symbol: | NDUFB8 |
| NCBI Official Synonym Symbols: | ASHI; CI-ASHI; MC1DN32 |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-ASHI; CI-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit |
| Protein Family: | |
| UniProt Gene Name: | NDUFB8 |
| UniProt Entry Name: | NDUB8_HUMAN |
| Antibodies |
| NDUFB8 Antibody (PACO10791) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
