Description
| 抗体名: | NDC80 Antibody (PACO23163) |
| 抗体コード: | PACO23163 |
| サイズ: | 100ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IF |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IF:1:100-1:500 |
| 反応性: | Human, Mouse |
| 免疫原: | Synthesized peptide derived from internal of human KNTC2. |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from Jurkat cells, using KNTC2 antibody. |
 | Immunofluorescence analysis of HUVEC cells, using KNTC2 antibody. |
| バックグラウンド: | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. |
| シノニム: | HEC1; HEC; Highly expressed in cancer; rich in leucine heptad repeats; KNTC2 |
| UniProt Protein Function: | HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 18p11.32 Cellular Component: chromosome, pericentric region; cytosol; kinetochore; membrane; nucleus Molecular Function:identical protein binding; protein binding; structural constituent of cytoskeleton Biological Process: attachment of spindle microtubules to kinetochore; attachment of spindle microtubules to kinetochore during mitosis; chromosome segregation; establishment of mitotic spindle orientation; mitosis; mitotic sister chromatid segregation; mitotic spindle organization and biogenesis; sister chromatid cohesion |
| NCBI Summary: | This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011] |
| UniProt Code: | O14777 |
| NCBI GenInfo Identifier: | 74735330 |
| NCBI Gene ID: | 10403 |
| NCBI Accession: | O14777.1 |
| UniProt Secondary Accession: | O14777,Q6PJX2, |
| UniProt Related Accession: | O14777 |
| Molecular Weight: | 73,913 Da |
| NCBI Full Name: | Kinetochore protein NDC80 homolog |
| NCBI Synonym Full Names: | NDC80, kinetochore complex component |
| NCBI Official Symbol: | NDC80 |
| NCBI Official Synonym Symbols: | HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80 |
| NCBI Protein Information: | kinetochore protein NDC80 homolog |
| UniProt Protein Name: | Kinetochore protein NDC80 homolog |
| UniProt Synonym Protein Names: | Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC |
| Protein Family: | Kinetochore protein |
| UniProt Gene Name: | NDC80 |
| UniProt Entry Name: | NDC80_HUMAN |
| Antibodies | ELISA Kits |
| Anti-NDC80 Antibody (CAB14102) | KNTC2 Colorimetric Cell-Based ELISA |
| Anti-NDC80 Antibody (CAB5411) | |
| Anti-HEC1 Antibody (CAB2392) | |
| NDC80 Antibody (PACO02313) | |
| NDC80 Antibody (PACO10767) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
