The NADK2 Polyclonal Antibody (PACO58184) is a valuable tool for researchers studying NADK2, a key enzyme involved in the regulation of cellular energy metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to the NADK2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in biochemistry and cellular metabolism research.
NADK2 plays a crucial role in the biosynthesis of NADP, a vital cofactor involved in numerous metabolic pathways. Dysregulation of NADK2 has been implicated in various diseases, including cancer and metabolic disorders. Understanding the function and regulation of NADK2 is essential for developing targeted therapies that could impact cellular metabolism and potentially lead to the identification of novel treatment strategies for these conditions.
Western Blot. Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue. All lanes: NADK2 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50, 46, 32 kDa. Observed band size: 50, 46 kDa.
IHC image of PACO58184 diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO58184 diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo.
Synonyms:
NAD kinase 2, mitochondrial (EC 2.7.1.23) (Mitochondrial NAD kinase) (NAD kinase domain-containing protein 1, mitochondrial), NADK2, C5orf33 MNADK NADKD1
UniProt Protein Function:
NADK2: Belongs to the NAD kinase family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: EC 2.7.1.23Chromosomal Location of Human Ortholog: 5p13.2Cellular Component: mitochondrionMolecular Function: NAD+ kinase activity; protein homodimerization activityBiological Process: NAD metabolic processDisease: 2,4-dienoyl-coa Reductase Deficiency
UniProt Protein Details:
NCBI Summary:
This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
