Description
| 抗体名: | MRPL12 Antibody (PACO16699) |
| 抗体コード: | PACO16699 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
| 反応性: | Human, Mouse |
| 免疫原: | Fusion protein of human MRPL12 |
| 憲法: | Liquid |
| ストレージバッファ: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| 精製方法: | Antigen affinity purification |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO16699(MRPL12 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200). |
 | Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: 293T cells, Primary antibody: PACO16699(MRPL12 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO16699(MRPL12 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200). |
| バックグラウンド: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. |
| シノニム: | mitochondrial ribosomal protein L12 |
| UniProt Protein Function: | MRPL12: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Ribosomal; Mitochondrial Chromosomal Location of Human Ortholog: 17q25 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial large ribosomal subunit Molecular Function:protein binding; structural constituent of ribosome; RNA binding Biological Process: mitochondrial translation; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; transcription from mitochondrial promoter |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P52815 |
| NCBI GenInfo Identifier: | 27436901 |
| NCBI Gene ID: | 6182 |
| NCBI Accession: | NP_002940.2 |
| UniProt Secondary Accession: | P52815,Q969U0, Q9HCA2, Q9UQJ3, |
| UniProt Related Accession: | P52815 |
| Molecular Weight: | 21,348 Da |
| NCBI Full Name: | 39S ribosomal protein L12, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L12 |
| NCBI Official Symbol: | MRPL12 |
| NCBI Official Synonym Symbols: | 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34 |
| NCBI Protein Information: | 39S ribosomal protein L12, mitochondrial; MRP-L12 |
| UniProt Protein Name: | 39S ribosomal protein L12, mitochondrial |
| UniProt Synonym Protein Names: | 5c5-2 |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL12 |
| UniProt Entry Name: | RM12_HUMAN |
