The Mouse TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit is a reliable and efficient tool for the accurate measurement of TRACP-5b levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.TRACP-5b is an important enzyme involved in bone metabolism, specifically in osteoclast activity and bone resorption. It serves as a marker for osteoclast number and function, making it valuable for studying bone-related conditions such as osteoporosis, arthritis, and other bone diseases.
By using the Mouse TRACP-5b ELISA Kit, researchers can easily analyze TRACP-5b levels in mouse samples, allowing for a better understanding of bone remodeling processes and the development of potential therapeutic strategies for bone disorders. Trust in this kit for accurate and dependable results in your research endeavors.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TRACP-5b. You can calculate the concentration of Mouse TRACP-5b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
91-104
88-100
Average (%)
98
97
93
1:4
Range (%)
89-104
85-98
82-92
Average (%)
95
90
87
1:8
Range (%)
90-103
84-97
87-102
Average (%)
97
90
94
1:16
Range (%)
92-103
87-98
85-95
Average (%)
98
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.52
5.33
22.43
2.7
4.86
21.67
Standard deviation
0.15
0.26
1.11
0.18
0.24
0.75
C V (%)
5.95
4.88
4.95
6.67
4.94
3.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TRACP-5b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TRACP-5b in samples. No significant cross-reactivity or interference between Mouse TRACP-5b and analogues was observed.
