Description
| Product Name: | Mouse TH Recombinant Protein |
| Product Code: | RPPB2316 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | TH |
| Synonyms: | Tyrosine3-monooxygenase, Tyrosine 3-hydroxylase, TH. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TH protein solution (0.25mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPMPTPSAS SPQPKGFRRAVSEQDTKQAE AVTSPRFIGR RQSLIEDARK EREAAAAAAA AAVASAEPGN PLEAVVFEER DGNAVLNLLFSLRGTKPSSL SRALKVFETF EAKIHHLETR PAQRPLAGSP HLEYFVRFEV PSGDLAALLS SVRRVSDDVRSAREDKVPWF PRKVSELDKC HHLVTKFDPD LDLDHPGFSD QAYRQRRKLI AEIAFQYKQG EPIPHVEYTKEEIATWKEVY ATLKGLYATH ACREHLEAFQ LLERYCGYRE DSIPQLEDVS HFLKERTGFQ LRPVAGLLSARDFLASLAFR VFQCTQYIRH ASSPMHSPEP DCCHELLGHV PMLADRTFAQ FSQDIGLASL GASDEEIEKLSTVYWFTVEF GLCKQNGELK AYGAGLLSSY GELLHSLSEE PEVRAFDPDT AAVQPYQDQT YQPVYFVSESFSDAKDKLRN YASRIQRPFS VKFDPYTLAI DVLDSPHTIR RSLEGVQDEL HTLTQALSAI SHHHHHH |
Tyrosine3-monooxygenase (Th), is a rate-limiting enzyme in catecholamine synthesis. Th utilizestetrahydrobiopterin as well as molecular oxygen to convert tyrosine toDOPA. Th regulates dopamine (DA)neurotransmission at the biosynthesis and reuptake steps. Th takes a vitalpart in the physiology of adrenergic neurons. Furthermore, Th effectsoverexpression in lymphocytes on the differentiation as well as function of Thelper cells.
TH Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 507 amino acids (1-498a.a.) and having a molecular mass of 57.0kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).�TH is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Vesicle; Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Mitochondrial; EC 1.14.16.2; Oxidoreductase Cellular Component: axon; cell soma; cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; cytosol; dendrite; internal side of plasma membrane; melanosome membrane; mitochondrion; neuron projection; nucleus; perikaryon; smooth endoplasmic reticulum; synaptic vesicle; terminal button Molecular Function:amino acid binding; dopamine binding; enzyme binding; ferric iron binding; ferrous iron binding; iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; oxygen binding; protein domain specific binding; tyrosine 3-monooxygenase activity Biological Process: aromatic amino acid family metabolic process; catecholamine biosynthetic process; dopamine biosynthetic process; dopamine biosynthetic process from tyrosine; eating behavior; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; eye photoreceptor cell development; heart development; learning; locomotory behavior; mating behavior; memory; neurotransmitter biosynthetic process; norepinephrine biosynthetic process; organ morphogenesis; regulation of heart contraction; response to ethanol; response to hypoxia; synaptic transmission, dopaminergic; synaptic vesicle amine transport; visual perception |
| UniProt Code: | P24529 |
| NCBI GenInfo Identifier: | 6678337 |
| NCBI Gene ID: | 21823 |
| NCBI Accession: | NP_033403.1 |
| UniProt Related Accession: | P24529 |
| Molecular Weight: | 55,993 Da |
| NCBI Full Name: | tyrosine 3-monooxygenase |
| NCBI Synonym Full Names: | tyrosine hydroxylase |
| NCBI Official Symbol: | Th�� |
| NCBI Protein Information: | tyrosine 3-monooxygenase |
| UniProt Protein Name: | Tyrosine 3-monooxygenase |
| UniProt Synonym Protein Names: | Tyrosine 3-hydroxylase; TH |
| Protein Family: | Tyrosine 3-monooxygenase |
| UniProt Gene Name: | Th�� |
| UniProt Entry Name: | TY3H_MOUSE |
