Description
| Product Name: | Mouse S100b Recombinant Protein |
| Product Code: | RPPB4572 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | S100b |
| Synonyms: | Protein S100-B, S-100 protein beta chain, S-100 proteinsubunit beta, S100 calcium-binding protein B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterilefiltered colorless solution. |
| Formulation: | The s100b protein solution (1mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSELEKAMVALIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDEDGDGECD FQEFMAFVAMVTTACHEFFE HE |
S100b is a member of the S100 family of proteins which are a family of EF-hand calcium binding proteins that exist mostly as dimers of the 20 currently identified individual S100 monomers. The S100B homodimer is expressed in cells of the central nervous system, glial cells and in certain peripheral cells e.g. Schwann cells, melanocytes, adipocytes and chondrocytes. S100 proteins are localized either in the cytoplasm or the nucleus of a wide range of cells. S100 proteins are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. There are at least 13 members in the S100 gene family, which are located as a cluster on chromosome 1q21; however, S100b is located at 21q22.3. The determination of S100B in serum levels may be used to monitor the extent of brain injury and malignant melanoma. S100b proteins may have a role in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of the S100b gene are implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
s100b Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 92 amino acids (1-92 a.a.) andhaving a molecular mass of 10.7kDa.The s100b is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. |
| UniProt Protein Details: | Protein type:Calcium-binding Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus Molecular Function:identical protein binding; RAGE receptor binding; protein homodimerization activity; zinc ion binding; metal ion binding; calcium ion binding; calcium-dependent protein binding; receptor binding Biological Process: regulation of long-term neuronal synaptic plasticity; cellular calcium ion homeostasis; regulation of cell shape; learning and/or memory; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; positive regulation of cell proliferation; energy reserve metabolic process; positive regulation of synaptic transmission; regulation of cytokine biosynthetic process; memory; regulation of neuronal synaptic plasticity |
| UniProt Code: | P50114 |
| NCBI GenInfo Identifier: | 6677839 |
| NCBI Gene ID: | 20203 |
| NCBI Accession: | NP_033141.1 |
| UniProt Related Accession: | P50114 |
| Molecular Weight: | 10,728 Da |
| NCBI Full Name: | protein S100-B |
| NCBI Synonym Full Names: | S100 protein, beta polypeptide, neural |
| NCBI Official Symbol: | S100b�� |
| NCBI Official Synonym Symbols: | Bpb; AI850290�� |
| NCBI Protein Information: | protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B |
| UniProt Protein Name: | Protein S100-B |
| UniProt Synonym Protein Names: | S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B |
| Protein Family: | Protein |
| UniProt Gene Name: | S100b�� |
| UniProt Entry Name: | S100B_MOUSE |
