The Mouse NT-proBNP (N-terminal pro-brain natriuretic peptide) ELISA Kit is specifically designed for the precise measurement of NT-proBNP levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable tool for a wide range of research purposes.NT-proBNP is a vital biomarker linked to heart failure and other cardiovascular conditions, providing valuable insights into cardiac function and prognosis.
By measuring NT-proBNP levels, researchers can gain a better understanding of cardiac health and assess the effectiveness of treatments and interventions.The Mouse NT-proBNP ELISA Kit is an essential resource for studying cardiovascular diseases and developing novel therapeutic approaches, offering reliable and reproducible data to advance scientific knowledge and improve patient outcomes.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NT-proBNP. You can calculate the concentration of Mouse NT-proBNP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
93-107
88-101
Average (%)
103
99
93
1:4
Range (%)
88-99
82-95
87-100
Average (%)
94
89
94
1:8
Range (%)
88-102
86-97
84-95
Average (%)
95
92
88
1:16
Range (%)
90-102
84-94
80-94
Average (%)
96
88
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.76
89.12
350.68
42.25
85.48
364.48
Standard deviation
2.86
5.07
11.12
2.11
3.44
18.26
C V (%)
6.25
5.69
3.17
4.99
4.02
5.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NT-proBNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NT-proBNP in samples. No significant cross-reactivity or interference between Mouse NT-proBNP and analogues was observed.
