Description
| Product Name: | Mouse MEP1B Recombinant Protein |
| Product Code: | RPPB3958 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | MEP1B |
| Synonyms: | MeprinA subunit beta (EC:3.4.24.63), Endopeptidase-2, Meprin B, Mep1b, Mep-1b. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | MEP1B protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% asdetermined by SDS-PAGE. |
| Amino Acid Sequence: | LPAPEKFVKDIDGGIDQDIF DINQGLGLDL FEGDIKLEAN GKNSIIGDHK RWPHTIPYVL EDSLEMNAKG VILNAFERYRLKTCIDFKPW SGEANYISVF KGSGCWSSVG NIHAGKQELS IGTNCDRIAT VQHEFLHALG FWHEQSRADRDDYVIIVWDR IQPGKEHNFN IYNDSVSDSL NVPYDYTSVM HYSKTAFQNG TESTIVTRIS EFEDVIGQRMDFSDYDLLKL NQLYNCTSSL SFMDSCDFEL ENICGMIQSS GDSADWQRVS QVLSGPESDH SKMGQCKDSGFFMHFNTSIL NEGATAMLES RLLYPKRGFQ CLEFYLYNSG SGNDQLNIYT REYTTGQQGG VLTLQRQIKEVPIGSWQLHY VTLQVTKKFR VVFEGLRGPG TSSGGLSIDD INLSETRCPH HIWHIQNFTQ ILGGQDTSVYSPPFYSSKGY AFQIYMDLRS STNVGIYFHL ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMFNQRSITTDPTMTSDNG SYFWDRPSKV GVTDVFPNGT QFSRGIGYGT TVFITRERLK SREFIKGDDI YILLTVEDISHLNSTSAVPD PVPTLAVHNA CSEVVCQNGG ICVVQDGRAE CKCPAGEDWW YMGKRCEKRG STRDVEHHHHHH |
Meprin A subunit beta (MEP1B) belongs to the astacin family of zinc endopeptidases 1, 2. MEP1B is a disulfide-linked, tetrameric metalloendopeptidase in renal brush border membranes. MEP1B is highly regulated, secreted cell-surface metalloendopeptidase, which is amply expressed in the kidney and intestine.
MEP1B produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 642 amino acids (21-654aa) and having a molecular mass of 72.6kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).MEP1Bis expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MEP1B: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]Protein type: EC 3.4.24.63; Membrane protein, integral; ProteaseCellular Component: membrane; integral to membrane; plasma membraneMolecular Function: peptidase activity; hydrolase activity; metallopeptidase activity; zinc ion binding; metal ion binding; metalloendopeptidase activityBiological Process: proteolysis; inflammatory response |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q61847 |
| NCBI GenInfo Identifier: | 147901863 |
| NCBI Gene ID: | 17288 |
| NCBI Accession: | NP_032612.2 |
| UniProt Secondary Accession: | Q61847,Q059K0 |
| UniProt Related Accession: | Q61847 |
| Molecular Weight: | 79,501 Da |
| NCBI Full Name: | meprin A subunit beta |
| NCBI Synonym Full Names: | meprin 1 beta |
| NCBI Official Symbol: | Mep1b�� |
| NCBI Official Synonym Symbols: | Mep-1b�� |
| NCBI Protein Information: | meprin A subunit beta; meprin B; meprin beta; endopeptidase-2 |
| UniProt Protein Name: | Meprin A subunit beta |
| UniProt Synonym Protein Names: | Endopeptidase-2; Meprin B |
| Protein Family: | Meprin |
| UniProt Gene Name: | Mep1b�� |
| UniProt Entry Name: | MEP1B_MOUSE |
