Description
| Product Name: | Mouse LYVE1 Sf9 Recombinant Protein |
| Product Code: | RPPB2562 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | LYVE1 Sf9 |
| Synonyms: | Lymphatic vessel endothelial hyaluronic acid receptor 1 precursor, LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1, Hyaluronic acid receptor, Extracellular link domain-containing protein. |
| Source: | Insect Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | LYVE1 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. |
| Solubility: | It is recommended to reconstitute the lyophilized LYVE1 in sterile water not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized sLYVE-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sLYVE-1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE. |
LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Soluble LYVE1 Mouse Recombinant fused to a C-terminal His-tag (6xHis) produced in baculovirus is a monomeric, glycosylated, polypeptide containing 228 amino acids (Met-1 to Gly 228) and having a molecular mass of 25 kDa but as a result of glycosilation the Mw is 40 kDa. The LYVE-1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 7|7 E3 Cellular Component: plasma membrane Molecular Function:hyaluronic acid binding; transmembrane receptor activity Biological Process: glycosaminoglycan catabolic process |
| UniProt Code: | Q8BHC0 |
| NCBI GenInfo Identifier: | 81873776 |
| NCBI Gene ID: | 114332 |
| NCBI Accession: | Q8BHC0.1 |
| UniProt Secondary Accession: | Q8BHC0,Q3TUC1, Q99NE4, |
| UniProt Related Accession: | Q8BHC0 |
| Molecular Weight: | 70 kDa |
| NCBI Full Name: | Lymphatic vessel endothelial hyaluronic acid receptor 1 |
| NCBI Synonym Full Names: | lymphatic vessel endothelial hyaluronan receptor 1 |
| NCBI Official Symbol: | Lyve1�� |
| NCBI Official Synonym Symbols: | Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik�� |
| NCBI Protein Information: | lymphatic vessel endothelial hyaluronic acid receptor 1 |
| UniProt Protein Name: | Lymphatic vessel endothelial hyaluronic acid receptor 1 |
| UniProt Synonym Protein Names: | Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1 |
| UniProt Gene Name: | Lyve1�� |
