Description
| Product Name: | Mouse LBP Recombinant Protein |
| Product Code: | RPPB3849 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | LBP |
| Synonyms: | Lipopolysaccharide-binding protein, LBP, Ly88. |
| Source: | Chinese Hamster Ovary Cells (CHO) |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Recombinant Mouse LBP was lyophilized from a protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2. |
| Solubility: | It is recommended to reconstitute the lyophilized LBP in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Biological Activity: | Up to 2�g/ml LBP mediates binding of FITC-LPS (0.5�g/ml) to CD14+CHO transfectants (2 x 106/ml). |
Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, and then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid.
LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE.�Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off.
| UniProt Protein Function: | LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 2 H1|2 78.72 cM Cellular Component: cell surface; extracellular space Molecular Function:lipopolysaccharide binding; receptor binding Biological Process: acute-phase response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; detection of molecule of bacterial origin; innate immune response; leukocyte chemotaxis during inflammatory response; lipopolysaccharide transport; lipopolysaccharide-mediated signaling pathway; macromolecule localization; macrophage activation during immune response; negative regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of cytolysis; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of macrophage activation; positive regulation of phagocytosis, engulfment; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; response to lipopolysaccharide |
| UniProt Code: | Q61805 |
| NCBI GenInfo Identifier: | 341940900 |
| NCBI Gene ID: | 16803 |
| NCBI Accession: | Q61805.2 |
| UniProt Secondary Accession: | Q61805,Q99KA0, A2AC66, |
| UniProt Related Accession: | Q61805 |
| Molecular Weight: | 53,055 Da |
| NCBI Full Name: | Lipopolysaccharide-binding protein |
| NCBI Synonym Full Names: | lipopolysaccharide binding protein |
| NCBI Official Symbol: | Lbp�� |
| NCBI Official Synonym Symbols: | Ly88; Bpifd2�� |
| NCBI Protein Information: | lipopolysaccharide-binding protein |
| UniProt Protein Name: | Lipopolysaccharide-binding protein |
| Protein Family: | Lipopolysaccharide-binding protein |
| UniProt Gene Name: | Lbp�� |
