Description
| Product Name: | Mouse IL33 Recombinant Protein |
| Product Code: | RPPB0666 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | IL33 |
| Synonyms: | 9230117N10Rik, Il-33, Il1f11, NF-HEV, Interleukin-33. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | IL33 protein solution (0.5mg/ml) containing Phosphate buffered saline,10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSSIQGTSL LTQSPASLST YNDQSVSFVL ENGCYVINVD DSGKDQEQDQ VLLRYYESPC PASQSGDGVD GKKLMVNMSP IKDTDIWLHA NDKDYSVELQ RGDVSPPEQA FFVLHKKSSD FVSFECKNLP GTYIGVKDNQ LALVEEKDES CNNIMFKLSK I |
| Biological Activity: | Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is ? 0.1ng/ml. |
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
IL33 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids (109-266 a.a) and having a molecular mass of 18.1kDa.IL33 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine Cellular Component: extracellular space; extracellular region; chromosome; cytoplasmic vesicle; nucleus Molecular Function:cytokine activity Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; defense response to virus; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response |
| UniProt Code: | Q8BVZ5 |
| NCBI GenInfo Identifier: | 68565902 |
| NCBI Gene ID: | 77125 |
| NCBI Accession: | Q8BVZ5.1 |
| UniProt Secondary Accession: | Q8BVZ5,Q2YEJ4, Q3TQN0, Q99L46, |
| UniProt Related Accession: | Q8BVZ5 |
| Molecular Weight: | 29,991 Da |
| NCBI Full Name: | Interleukin-33 |
| NCBI Synonym Full Names: | interleukin 33 |
| NCBI Official Symbol: | Il33�� |
| NCBI Official Synonym Symbols: | Il-33; Il1f11; NF-HEV; 9230117N10Rik�� |
| NCBI Protein Information: | interleukin-33; nuclear factor from high endothelial venules |
| UniProt Protein Name: | Interleukin-33 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il33�� |
| UniProt Entry Name: | IL33_MOUSE |
