Description
| Product Name: | Mouse IL3 Recombinant Protein |
| Product Code: | RPPB0499 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | IL3 |
| Synonyms: | Interleukin-3, Interleukin-3, sf9, IL3 sf9, L-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor, Il3, Csfmu, Il-3, BPA, Csfmu, HCGF, Il-3, MCGF, PSF. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | IL3 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ASISGRDTHR LTRTLNCSSI VKEIIGKLPE PELKTDDEGP SLRNKSFRRV NLSKFVESQG EVDPEDRYVI KSNLQKLNCC LPTSANDSAL PGVFIRDLDD FRKKLRFYMV HLNDLETVLT SRPPQPASGS VSPNRGTVEC LEHHHHHH |
| Biological Activity: | The ED50 is Measured in a cell proliferation assay using TF-1 human erythroleukemic and is ? 0.1 ng/ml. |
Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
IL3 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 148 amino acids (27-166 a.a.) and having a molecular mass of 16.7kDa (Migrates at 18-28kDa on SDS-PAGE under reducing conditions).IL3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Cytokine; Apoptosis; Secreted, signal peptide; Secreted; Oncoprotein Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-3 receptor binding; growth factor activity; cytokine activity Biological Process: myeloid cell apoptosis; regulation of protein amino acid dephosphorylation; negative regulation of autophagy; cytokine and chemokine mediated signaling pathway; response to hormone stimulus; positive regulation of JNK cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of gene expression; negative regulation of mast cell apoptosis; regulation of glycolysis; positive regulation of cell proliferation; response to hypoxia; hemopoiesis; immune response; regulation of cell growth; positive regulation of protein amino acid phosphorylation; positive regulation of myeloid leukocyte differentiation; negative regulation of apoptosis |
| UniProt Code: | P01586 |
| NCBI GenInfo Identifier: | 124333 |
| NCBI Gene ID: | 16187 |
| NCBI Accession: | P01586.1 |
| UniProt Related Accession: | P01586 |
| Molecular Weight: | 18,540 Da |
| NCBI Full Name: | Interleukin-3 |
| NCBI Synonym Full Names: | interleukin 3 |
| NCBI Official Symbol: | Il3�� |
| NCBI Official Synonym Symbols: | BPA; PSF; HCGF; Il-3; MCGF; Csfmu�� |
| NCBI Protein Information: | interleukin-3; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor |
| UniProt Protein Name: | Interleukin-3 |
| UniProt Synonym Protein Names: | Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il3�� |
| UniProt Entry Name: | IL3_MOUSE |
