The Mouse IL-3 CLIA Kit is a high-quality assay designed for the precise measurement of interleukin-3 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs.Interleukin-3 is a key cytokine that plays a crucial role in the regulation of hematopoiesis and immune response. Its dysregulation has been linked to various inflammatory and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing therapeutic strategies.
With easy-to-follow protocols and quick assay turnaround times, the Mouse IL-3 CLIA Kit is an indispensable tool for researchers looking to unravel the complexities of immune function and inflammation in mouse models. Order yours today and advance your research with confidence.
Product Name:
Mouse IL-3 CLIA Kit
SKU:
MOES00402
Target:
Mouse IL-3
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-3. You can calculate the concentration of Mouse IL-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
102-117
83-97
Average (%)
93
110
90
1:4
Range (%)
95-107
87-102
85-97
Average (%)
101
94
91
1:8
Range (%)
95-110
98-115
97-112
Average (%)
103
105
103
1:16
Range (%)
90-102
88-102
85-96
Average (%)
95
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
95-113
103
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.58
59.49
233.23
26.97
58.55
216.89
Standard deviation
2.1
4.75
22.95
2.18
4.87
14.29
C V (%)
8.21
7.98
9.84
8.08
8.32
6.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-3 in samples. No significant cross-reactivity or interference between Mouse IL-3 and analogues was observed.
