Description
| Product Name: | Mouse IL 1 alpha Recombinant Protein |
| Product Code: | RPPB0447 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | IL 1 alpha |
| Synonyms: | Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 1xPBS, pH=7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin 1a in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS |
| Biological Activity: | The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.002 ng/ml. |
Interleukin-1 alpha is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. Circulating levels of are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1 alpha possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.
Interleukin-1A Mouse Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 156 amino acids and having a molecular mass of 17.9 kDa. The IL-1A is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytokine Cellular Component: extracellular space; cell surface; plasma membrane; extracellular region; cytosol Molecular Function:interleukin-1 receptor binding; copper ion binding; cytokine activity Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; positive regulation of interleukin-6 production; fever; positive regulation of stress-activated MAPK cascade; negative regulation of cell proliferation; positive regulation of angiogenesis; connective tissue replacement during inflammatory response; response to copper ion; positive regulation of cell division; keratinization; positive regulation of prostaglandin secretion; immune response; positive regulation of transcription from RNA polymerase II promoter; regulation of sensory perception of pain; inflammatory response; positive regulation of cytokine secretion |
| UniProt Code: | P01582 |
| NCBI GenInfo Identifier: | 124298 |
| NCBI Gene ID: | 16175 |
| NCBI Accession: | P01582.1 |
| UniProt Related Accession: | P01582 |
| Molecular Weight: | 31,023 Da |
| NCBI Full Name: | Interleukin-1 alpha |
| NCBI Synonym Full Names: | interleukin 1 alpha |
| NCBI Official Symbol: | Il1a�� |
| NCBI Official Synonym Symbols: | Il-1a�� |
| NCBI Protein Information: | interleukin-1 alpha; IL-1 alpha |
| UniProt Protein Name: | Interleukin-1 alpha |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il1a�� |
| UniProt Entry Name: | IL1A_MOUSE |
