Description
| Product Name: | Mouse GSTA1 Recombinant Protein |
| Product Code: | RPPB1757 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | GSTA1 |
| Synonyms: | Glutathione S-transferase A1, GST class-alpha member 1,Glutathione S-transferase Ya , Glutathione S-transferase Ya1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GSTA1 protein solution (1mg/ml) containing Phosphatebuffered saline (pH7.4), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAGKPVL HYFNARGRME CIRWLLAAAG VEFEEKFIQS PEDLEKLKKDGNLMFDQVPM VEIDGMKLAQ TRAILNYIAT KYDLYGKDMK ERALIDMYSE GILDLTEMIG QLVLCPPDQREAKTALAKDR TKNRYLPAFE KVLKSHGQDY LVGNRLTRVD IHLLEVLLYV EEFDASLLTP FPLLKAFKSRISSLPNVKKF LQPGSQRKPP MDAKQIQEAR KAFKIQ |
| Biological Activity: | The specific activity is defined as the amount of enzyme that conjugate 1.0 pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C and is > 4,000 pmol/min/ug. |
Membrane-bound & Cytosolic forms of GST are encoded by 2 separate supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. There are 8 different classes of soluble cytoplasmic mammalian GST: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTA1 is found in a cluster mapped to chromosome 6, and is highly expressed in the liver. GSTA1 protects the cells from reactive oxygen species.
GSTA1 Mouse Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 246 amino acids (1-223 a.a) andhaving a molecular mass of 28kDa.GSTA1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GSTA5: Belongs to the GST superfamily. Alpha family. Homodimer |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Nuclear envelope; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450 Molecular Function:glutathione transferase activity Biological Process: glutathione metabolic process |
| UniProt Code: | P13745 |
| NCBI GenInfo Identifier: | 154350202 |
| NCBI Gene ID: | 14857 |
| NCBI Accession: | NP_032207.3 |
| UniProt Secondary Accession: | P13745,A2RTN4, |
| UniProt Related Accession: | P13745 |
| Molecular Weight: | 26.7kDa |
| NCBI Full Name: | glutathione S-transferase A1 |
| NCBI Synonym Full Names: | glutathione S-transferase, alpha 1 (Ya) |
| NCBI Official Symbol: | Gsta1�� |
| NCBI Official Synonym Symbols: | Gst2-1; OTTMUSG00000031890�� |
| NCBI Protein Information: | glutathione S-transferase A1; GST class-alpha member 1; glutathione S-transferase Ya; glutathione S-transferase Ya1 |
| UniProt Protein Name: | Glutathione S-transferase A1 |
| UniProt Synonym Protein Names: | GST class-alpha member 1; Glutathione S-transferase Ya; Glutathione S-transferase Ya1 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | Gsta1�� |
| UniProt Entry Name: | GSTA1_MOUSE |
