Description
system_update_altDatasheet
| Product SKU: | SBRS1376 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse Amphiregulin, Axl, CD27 Ligand (TNFSF7), CD30 (TNFRSF8), CD40 (TNFRSF5), CXCL16, EGF, E-Selectin, Fractalkine (CX3CL1), HGF, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ra (IL-1 F3), IL12 p70, IL-17E (IL-25), IL-17F, IL-2 R alpha, IL-20, IL-23 p19, IL-28A (IFN-lambda 2), I-TAC (CXCL11), MDC (CCL22), MIP2, MIP-3 alpha (CCL20), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Prolactin, Pro-MMP-9, PSelectin, Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Thrombopoietin (TPO), VCAM-1 (CD106), VEGF-A, VEGF-D. |
| Uniprot: | O35714 |
| Gene ID: | 21936 |
| Gene Names: | GITR / TNFRSF18 |
| Synonyms: | Tumor necrosis factor receptor superfamily member 18 (Glucocorticoid-induced TNFR-related protein) (CD antigen CD357) |
| Target Species: | Mouse |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.03 ng/ml - 10 ng/ml |
| Sensitivity: | 0.03 ng/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Mouse GITR/TNFRSF18/AITR PharmaGenie ELISA Kit (SBRS1376) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
