Description
| Product Name: | Mouse Fractalkine Recombinant Protein |
| Product Code: | RPPB1139 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | Fractalkine |
| Synonyms: | Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, Cx3cl1, Cx3c, Fkn, Scyd1, CX3C, ABCD-3, AB030188, AI848747, D8Bwg0439e. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized CX3CL1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized CX3CL1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CX3CL1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNG |
| Biological Activity: | The ED50as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 �g/ml, corresponding to a specific activity of > 2000IU/mg. |
Fractalkine soluble form is chemotactic for t-cells and monocytes, but not for neutrophils. Fractalkine membrane-bound form promotes adhesion of those leukocytes to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373-amino acid) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the cell surface. Fractalkine gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.
Fractalkine Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8.7kDa. The CX3CL1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis Cellular Component: extracellular space; cell surface; cell projection; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; integral to membrane; extracellular region Molecular Function:chemokine activity; cytokine activity Biological Process: platelet activation; positive regulation of transforming growth factor-beta1 production; neutrophil chemotaxis; positive regulation of neuroblast proliferation; positive regulation of cell adhesion; wound healing; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; negative regulation of cytokine secretion; microglial cell activation; chemotaxis; angiogenesis involved in wound healing; leukocyte adhesive activation; macrophage chemotaxis; negative regulation of vasoconstriction; regulation of inhibitory postsynaptic membrane potential; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of cell migration |
| UniProt Code: | O35188 |
| NCBI GenInfo Identifier: | 342187356 |
| NCBI Gene ID: | 20312 |
| NCBI Accession: | O35188.3 |
| UniProt Secondary Accession: | O35188,O35933, Q91V44, |
| UniProt Related Accession: | O35188 |
| Molecular Weight: | 42,099 Da |
| NCBI Full Name: | Fractalkine |
| NCBI Synonym Full Names: | chemokine (C-X3-C motif) ligand 1 |
| NCBI Official Symbol: | Cx3cl1�� |
| NCBI Official Synonym Symbols: | CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e�� |
| NCBI Protein Information: | fractalkine; neurotactin; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D, 1 |
| UniProt Protein Name: | Fractalkine |
| UniProt Synonym Protein Names: | C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1Cleaved into the following chain:Processed fractalkine |
| Protein Family: | Fractalkine |
| UniProt Gene Name: | Cx3cl1�� |
| UniProt Entry Name: | X3CL1_MOUSE |
