Mouse FNDC5 (Fibronectin type III domain-containing protein 5) ELISA kit
The Mouse FNDC5 (Fibronectin Type III Domain-Containing Protein 5) ELISA Kit is a powerful tool for the precise measurement of FNDC5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.FNDC5 is a key protein involved in metabolic regulation and exercise-induced hormone production, particularly in the context of regulating energy expenditure and promoting brown adipose tissue activation.
Dysregulation of FNDC5 has been implicated in metabolic disorders, making it a valuable target for studying these conditions and developing potential treatments.Order your Mouse FNDC5 ELISA Kit today to unlock new insights into the role of FNDC5 in metabolism and metabolic diseases.
Product Name:
Mouse FNDC5 (Fibronectin type III domain-containing protein 5) ELISA kit
SKU:
MOES00945
Target:
Mouse FNDC5 (Fibronectin type III domain-containing protein 5) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FNDC5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FNDC5. You can calculate the concentration of Mouse FNDC5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
88-99
94-107
Average (%)
103
93
101
1:4
Range (%)
91-102
81-92
88-104
Average (%)
96
87
95
1:8
Range (%)
92-109
84-96
85-99
Average (%)
100
89
91
1:16
Range (%)
93-103
88-99
82-96
Average (%)
98
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
194.11
575.25
1915.16
202.17
619.86
2032.21
Standard deviation
12.64
31.7
63.97
12.78
30.81
68.89
C V (%)
6.51
5.51
3.34
6.32
4.97
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FNDC5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FNDC5 in samples. No significant cross-reactivity or interference between Mouse FNDC5 and analogues was observed.
